产品货号：
Z23274
中文名称：
PDHB抗体
英文名称：
Anti-PDHB antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-PDHB antibody |
| 指标别名 | PDHB;PDHE 1B;PDHE1 B;PDHE1B;PHE1B;Pyruvate Dehydrogenase E1 beta subunit |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,IP,ELISA |
| 基因名称 | PDHB |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human PDHB recombinant protein (Position:M1-K354). |
| 实际分子量 | 34KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide,and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle.The PDH complex is composed of multiple copies of three enzymatic components:pyruvate dehydrogenase (E1),dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).The E1 enzyme is a heterotetramer of two alpha and two beta subunits.This gene encodes the E1 beta subunit.Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency.Alternatively spliced transcript variants have been found for this gene. |
| Uniprot ID | P11177 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P) and ICC. |
| 基因名全称 | pyruvate dehydrogenase E1 subunit beta |
| 蛋白名全称 | Pyruvate dehydrogenase E1 component subunit beta,mitochondrial |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 ImmunoPrecipitation (IP) 1:50 Enzyme linked immunosorbent assay (ELISA) 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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